in-vitro callus propagation and secondary metabolite quantification in sericostoma pauciflorum

نویسندگان

satish c. jain medicinal plants and biotechnology laboratory, department of botany, university of rajasthan, jaipur-302004, india.

boskey pancholi medicinal plants and biotechnology laboratory, department of botany, university of rajasthan, jaipur-302004, india.

renuka jain department of chemistry, university of rajasthan, jaipur-302004, india.

چکیده

sericostoma pauciflorum stocks ex wight(family boraginaceae) used against cancer, diabetes and known to be health promoter. callus cultures have been established from the stem explants on murashige and skoog (ms) medium supplemented with 1.5 mg/l concentration of different growth hormone viz. kinetin (kn), indole 3-acetic acid (iaa) and indole 3-butyric acid (iba). at 6 weeks of age, these calli were harvested, dried and extracted successively in pet, ether, methanol and water. extracts were dried, weighed (%) and analyzed for their bioefficacies. antimicrobial activities were determined using agar well diffusion and antioxidant potentials by dpph and frap methods. among all the test extracts, the extract of stem callus raised on iba found to be more effective whereas it’s pet. ether extract showed appreciable activity against both the test bacteria and fungi ( s. aureus- iz 14.00 ± 0.57 mm and t. rubrum- 16.33 ± 0.32 mm), followed by methanol extract ( s. aureus- iz 13.00 ± 0.57 mm, a. niger and p. chrysogenum - iz 16.66 mm in both). in antioxidant potentials, all aqueous extracts were more active where iba and kn extracts demonstrated 0.06 mg/ml ic50 value (% inhibition 93.30 and 92.70 respectively at 0.8 mg/ml concentration) with 366 ± 6.69 and 343 ± 3.34 ascorbic acid equivalent antioxidant potentials at 1 mg/ml concentration. furthermore, the chemical profile of test extracts was carried out. the bioactive secondary metabolites, β -sitosterol and caffeic acid was isolated from culture tissue of 6 weeks-old callus, and their identification and confirmation was carried out by color reaction, tlc behavior and ir spectrum techniques.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum

Sericostoma pauciflorum Stocks ex Wight(Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinetin (Kn), indole 3-acetic acid (IAA) and indole 3-butyric acid (IBA). At 6 weeks of age, these call...

متن کامل

In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum

Sericostoma pauciflorum Stocks ex Wight(Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinetin (Kn), indole 3-acetic acid (IAA) and indole 3-butyric acid (IBA). At 6 weeks of age, these call...

متن کامل

In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum

Sericostoma pauciflorum Stocks ex Wight (Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinetin (Kn), indole 3-acetic acid (IAA) and indole 3-butyric acid (IBA). At 6 weeks of age, these cal...

متن کامل

In vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...

متن کامل

In vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...

متن کامل

Secondary metabolite content in rhizomes, callus cultures and in vitro regenerated plantlets of Solidago chilensis.

An in vitro culture system leading to the formation of callus and plant regeneration, starting from nodal sections and shoot tips, was developed for Solidago chilensis (Asteraceae). The content of the gastroprotective diterpene solidagenone as well as the phenolics chlorogenic acid (CA) and rutin was determined either in rhizomes from wild growing plants and in callus and in in vitro regenerate...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
the iranian journal of pharmaceutical research

جلد ۱۱، شماره ۴، صفحات ۱۱۰۳-۱۱۰۹

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023